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Literature summary for 2.7.7.B22 extracted from

  • Inaba, Y.; Banerjee, I.; Kernan, T.; Banta, S.
    Transposase-mediated chromosomal integration of exogenous genes in Acidithiobacillus ferrooxidans (2018), Appl. Environ. Microbiol., 84, e01381 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology application of a hyperactive transposase variant to generate mutants with integrated genes for the expression of the superfolder green fluorescent protein gene or a 2-ketodecarboxylase gene in Acidithiobacillus ferrooxidans, which enables the production and secretion of isobutyric acid. An inverse PCR method identifies the insertion sites of the 2-ketodecarboxylase gene, i.e. functional exogenous metabolic genes have been chromosomally integrated Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression in Acidithiobacillus ferrooxidans Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli Q46731
-
-

Synonyms

Synonyms Comment Organism
Tn5
-
Escherichia coli
TnpA
-
Escherichia coli