Application | Comment | Organism |
---|---|---|
biotechnology | application of a hyperactive transposase variant to generate mutants with integrated genes for the expression of the superfolder green fluorescent protein gene or a 2-ketodecarboxylase gene in Acidithiobacillus ferrooxidans, which enables the production and secretion of isobutyric acid. An inverse PCR method identifies the insertion sites of the 2-ketodecarboxylase gene, i.e. functional exogenous metabolic genes have been chromosomally integrated | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression in Acidithiobacillus ferrooxidans | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q46731 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
Tn5 | - |
Escherichia coli |
TnpA | - |
Escherichia coli |